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Methods of Artificial Insemination
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VAGINAL ARTIFICIAL INSEMINATION
The bitch stands on an
inclined, non-slip ramp, head down, to facilitate the gravitational
flow of semen to the opening of the cervix. The semen is
inseminated from a syringe through a plastic rod. This method is
best used in situations where both the male and female are deemed
reproductively healthy and a natural breeding is not desirable or
attainable.
A major advantage of this method is that it allows evaluation of
the male's semen, not easily done after a natural breeding. This
technique does not require any sedation, only manual restraint.
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INTRA-UTERINE SEMEN DEPOSITION
The advantage of depositing semen in the uterus
was first realized when canine frozen was first utilized.
Conception rates increased six times when the canine frozen semen
was deposited in the uterus rather than vaginally. Two methods of
intra-uterine semen deposition are currently being performed at
Brownsville/Lomira Small Animal Clinic /International Canine Semen
Bank-Wisconsin.
TRANSCERVICAL INSEMINATION
uses a rigid endoscope to visualize the cervical
opening of the bitch. A flexible plastic catheter is guided through
the cervix into the uterus, where the semen is deposited. The
technique does not require sedation and causes no damage to the
vaginal tract, cervix, or uterus.
The drawback is that the uterine
lining, the weak spot in the bitch's reproduction cycle, cannot be
evaluated. This technique is best used on younger bitches (less
than three years of age) with no suspected uterine damage when
using compromised quality fresh-cooled or frozen semen.
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SURGICAL INTRAUTERINE DEPOSITION OF SEMEN
gives a bitch the greatest chance of conception. The procedure allows for the examination of the uterus for cysts, occlusions and adhesions. Many times, corrections of any problems are possible at the time of the surgery, greatly increasing a bitch's chance of conception.
The procedure does require anesthesia. Propofol(R), a short-acting injectable anesthetic, is given. The bitch is then intubated and placed on Sevothane(R), a gas anesthetic.
A 2-3 inch abdominal incision is performed, then the uterus is isolated and evaluated and the semen injected into the uterus through a hypodermic needle. No incision is made in the uterus. The muscle and skin are sutured. The total technique takes approximately 15-30 minutes. The bitch is sent home immediately after the surgery.
When using frozen semen, surgical insemination is used. This ensures this compromised semen is delivered to the uterus, close to the oviducts where fertilization takes place. Surgical insemination is also useful when breeding a reproductively older bitch (four years or older) or using semen that is of lower quality.
The basis of artificial insemination is to enhance the bitch's chance of conceiving and present you with the puppies you desire. Dr. Marty Greer and her highly trained staff will be more than happy to consult with you concerning the best method of artificial insemination for your individual bitch. |
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The litter above was born 1 year after the
mother was treated for Pyometra |
Pellets of frozen semen
ready for packaging |
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Dr. Greer's litter of 10 Pembroke Corgis |
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